Infection is a major contributing factor to delayed healing in chronic wounds. The misuse of antimicrobials is common to all specialties, and recent publications have highlighted the importance of antimicrobial stewardship in wound care. In view of this, the authors audited the prescription of systemic and topical antimicrobials as well as microbiological sampling practices in a multidisciplinary complex wound clinic. Only 9.3% of patient encounters resulted in the use of systemic antibiotics, whereas topical antimicrobials were used more frequently. Microbiological sampling was rarely performed, and swabbing was done more often than tissue biopsy.